Growth state-dependent phenotypes of adult hepatocytes in primary monolayer culture.
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چکیده
منابع مشابه
Adult rat hepatocytes in primary monolayer culture. Ultrastructural characteristics of intercellular contacts and cell membrane differentiations
Primary monolayer cultures were obtained in 60-mm petri dishes by incubating 3 X 10(6) isolated hepatocytes at 37 degrees C in Dulbecco's medium supplemented with 17% fetal calf serum. The ultrastructure of monolayer cells was examined after various incubation periods. Within 4 h of plating, the isolated spherical cells adhere to the plastic surface, establish their first contacts by numerous i...
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The initiation of DNA synthesis has been studied in quiescent primary cultures of fetal rat hepatocytes using defined hormones and chemically defined medium. Preparations of crystalline insulin (0.01-10 microg/ml) or 20,000-fold purified somatomedin (0.01-1 microg/ml) are stimulatory. Growth hormone (0.025 microg/ml) and hydroxycortisone (0.025 microg/ml), 3':5'-GMP! (10(-5) M) fail by themselv...
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We have studied the effect of 3,5,3'-triiodothyronine (T3) on the respiration of adult rat hepatocytes in primary monolayer culture prepared from hypothyroid rat liver. After addition of T3 to the culture medium at a concentration of 2 x 10(-7) M, oxygen consumption of the cultured cells increased detectably at 24 h and was maximal at 72--96 h, relative to control cultures (38.0 +/- 1.8 vs. 25....
متن کاملThyroid Thermogenesis in Adult Rat Hepatocytes in Primary Monolayer Culture Direct Action of Thyroid Hormone In Vitro
We have studied the effect of 3,5,3 't r i iodothyronine (Ts) on the respiration of adul t rat hepatocytes in pr imary monolayer culture p repared f rom hypothyroid rat liver. After addit ion of T3 to the culture medium at a concentration of 2 x 10 -7 M, oxygen consumption of the cultured cells increased detectably at 24 h and was maximal at 72-96 h, relative to control cultures (38.0 -+ 1.8 vs...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1978
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.75.4.1834